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KMID : 0370219780220010015
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Yakhak Hoeji
1978 Volume.22 No. 1 p.15 ~ p.21
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Studies on the Fluorophotometric Determination of Gentamicin Sulfate and its Preparations
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백우현/Paik WH
김정우/허명권/박근창/Kim JW/Hou MK/Park KC
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Abstract
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Gentamicin sulfate reacted with pyridoxal and zinc(II) ion in pyridine-methanol solution to yield highly fluorescent zinc(II) chelates of N-pyridoxylidene derivatives. This fluorescence reaction was sensitive and showed excitation maximum at 398nm, and emission maximum at 482nm. The effects of reagent concentration, reaction time and temperature, standing time and temperature were studied. And a new fluorophotometric method for the determination of gentamicin sulfate was developed. A good result was obtained and this method was applied to various preparations.
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